Table 2.
Activities of formate dehydrogenase and nitrate reductase are ErpA and IscA dependenta
| Strainb | Sp act of Fdh-N (U mg of protein−1)c | Sp act of Nar (U mg of protein−1)c |
|---|---|---|
| MC4100 | 0.15 ± 0.08 | 0.40 ± 0.09 |
| MG1655 | 0.15 ± 0.03 | 0.55 ± 0.40 |
| BW25113 | 0.13 ± 0.04 | 0.55 ± 0.16 |
| JW1215 (ΔnarG) | 0.21 ± 0.12 | <0.01 |
| JW1470 (ΔfdnG) | 0.03 ± 0.03 | 0.43 ± 0.30 |
| CP477 (ΔiscA) | 0.08 ± 0.03 | 0.08 ± 0.04 |
| CP477 (ΔiscA)(piscA) | 0.37 ± 0.29 | 0.32 ± 0.12 |
| CP477 (ΔiscA)(perpA) | 0.08 ± 0.01 | 0.02 ± 0.01 |
| LL402 (ΔerpA) | <0.01 | 0.02 ± 0.02 |
| LL401 (erpA) | 0.01 ± 0.01 | 0.02 ± 0.02 |
| LL401 + l-arabinose | 0.10 ± 0.06 | 0.3 ± 0.08 |
| LL401(piscA) | 0.01 ± 0.01 | 0.11 ± 0.04 |
| LL401(piscA) + l-Ara | 0.07 ± 0.01 | 0.27 ± 0.05 |
| LL401(perpA)d | 0.52 ± 0.14 | 0.24 ± 0.03 |
| CP1223 (sufA) | 0.71 ± 0.29 | 1.19 ± 0.25 |
| DV1151 (ΔiscA ΔerpA) | <0.01 | 0.02 ± 0.03 |
| JW2513 (iscU) | <0.01 | 0.01 ± 0.04 |
a
Cells were grown anaerobically in TGYEP medium (pH 6.5) supplemented with 100 mM KNO3.
b
The strain, relevant genotype, whether it carries a plasmid (e.g., plasmid piscA or perpA), and whether the strain was grown with l-arabinose is shown.
c
The means ± standard errors of the means of at least three independent experiments are shown.
d
Performed with two different plasmids encoding ErpA (perpA and pLUE-A) with both delivering similar results.