Table 1.
Strains and primers
| Strain or primer | Characteristics/sequence | Reference or source |
|---|---|---|
| P. mirabilis strains | ||
| BB2000 | Wild type; spontaneous Rfr from PRM1 | 13 |
| BB2204 | BB2000 fliL::Tn5-Cm | 16 |
| YL1001 | Motile revertant of BB2204; Cms | This study |
| Primers | ||
| fliLpF | GGATCCGTGGTGTCGATATTT | |
| fliL_r | GAGCATCTATCTCTGCCTGTGAAAGG | |
| rpoAF | GCGTGTTATAGCCCAGTTGA | |
| rpoAR | AGGCTGACGAACATCACGTA | |
| flhDF | AAGGCTTCCGCAATGTTTAGAC | |
| flhDR | GTTGCAAATCATCCACTCTGGA | |
| fliAF | CCTGCGAGTGTTGAATTGGA | |
| fliAR | GGATTGTGTCACTTCTCTTGC | |
| fliLF | GGTGATCGCCATTATTGCAG | |
| fliLR | AGCGTAACGTGATCCCTATG | |
| fliMF | GTCAATTCCGTATGGGGCTG | |
| fliMR | AACCAGATTCGGCTCAAAGG | |
| flgMF | CGCACAAATCCACTTATCCC | |
| flgMR | GGGCAACTTTTTCGACATTG | |
| flaAF | CAACTGAAGGTGCATTGAAC | |
| flaAR | TGATTTCTCACCGCTCAGTA | |
| motAF | GATGGTGACGGGGAATATGAA | |
| motAR | CCATTTCCCCAGCAGGTCTA | |
| umoAF | TCCACCACCACCACACGTAA | |
| umoAR | CGCAATCCTTTGCCTGTCCTA | |
| umoBF | TATATGCCTCCAAACCTTTCCA | |
| umoBR | AGCTTGGGTTTACACGGTTG | |
| umoCF2 | CTCTTTTTCCTCCTTTGCTCA | |
| umoCR2 | CACTATCTTCAAGGTCGCCT | |
| umoDF | CAAGAGTGCCGTGTTTTCTATA | |
| umoDR | CGATGATATCGCCCGGTTTAA | |
| wosAF2 | GCCCCTTATGCTGTCATGAA | |
| wosAR2 | GCCATTCAAAATCTGGTCACG | |
| rcsFF | CTCCTGTTCGCATTGTTGAA | |
| rcsFR | TTCCTGTGACTATTTGGCATTC | |
| rsbAF | CTATACCTACCGCACCATGT | |
| rsbAR | GAAGTCCCATCCGTTGATAC | |
| rcsBF | CGCCTGCTGATCTACCTAAA | |
| rcsBR | GCAATCTCAGTGACAAGGAA | |
| rcsCF | CGGCTACCCACATTTTCTTGA | |
| rcsCR | CCGGCAGAGGGATAAATGAAA | |
| mrpAF | TGATGCTCCTTGCTCAATTAC | |
| mrpAR | AGTTGCTTCAGAGCCAGTGA | |
| ucaJF | GGAGGGAAATAGCCCATTCA | |
| ucaJR | TTGGCATAATGCTTTCTGCTGA | |
| pmpJF | CTTGATGTGAGTCAGCAACA | |
| pmpJR | CACGCAAATAGAATGGAAACG | |
| hpmBF | GGCGTTGAATGGCTAAGTTTA | |
| hmpBR | CCACCTTGCCATCCTTTGTA | |
| zapAF | GGCCAAGCATGGTTTAGTGA | |
| zapAR | GGCGACTATCTTCCGCATAA |