Fig 1.

Growth and OMP phenotypes of various bam mutants. (A) Growth of wild type (WT), ΔbamB, ΔbamE, and ΔbamB ΔbamE strains on minimal and rich media was assessed by the single-colony isolation method. Plates were incubated for 24 (rich medium) or 36 h (minimal medium) at 30°C. (B) OMPs were detected from whole-cell lysates prepared from overnight cultures grown in glycerol minimal liquid medium at 30°C by Western blot analysis. Each lane contains protein samples from equal number of cells (based on the optical density at 600 nm). Membrane blots were probed with antibodies specific to OmpA, OmpC, OmpF, LamB, and GroEL. GroEL served as a gel loading control.