Fig 3.

Determination of BamA levels from different genetic backgrounds. BamA levels were determined by Western blotting of whole-cell lysates prepared from cultures grown overnight in glycerol minimal liquid medium at 30°C. Membrane blots containing protein samples from control strains (A) or BamA mutants (B) were probed with antibodies specific to BamA and AcrA, with the latter serving as a gel loading control. BamA levels were determined relative to the gel loading control, AcrA, and then normalized to the wild-type value of 1.