Fig 4.

Assessment of BamA's heat-modifiability property in different genetic backgrounds. Envelopes obtained from strains grown overnight in glycerol minimal liquid medium at 30°C were extracted with 0.1 M sodium carbonate at 4°C for 1 h. Sodium carbonate-insoluble membrane pellets were solubilized in an SDS buffer. Prior to SDS-PAGE analysis, SDS-solubilized samples either were left at room temperature (25°C) or heated (100°C). The detection of BamA was carried out by Western blot analysis using BamA-specific antibodies. Relevant genetic backgrounds of strains are labeled. BamA in four control strains (A), BamA mutants in a ΔbamB ΔbamE background (B), and three representative BamA mutants in a BamB⁺ BamE⁺ background (C) are shown.