Fig 6.

Effects of BamA β-barrel alterations on growth and OMP phenotypes in a ΔsurA background. (A) Growth of various strains on Luria broth agar after 24 h of incubation at 30 and 37°C. The relevant genotypes of the strains are shown. (B) Determination of OMP and AcrA levels by Western blot analysis, as described in the legend to Fig. 1. LamB levels were calculated relative to AcrA, a gel loading control, and normalized to the wild-type value of 1. (C) Examination of the BamA's heat-modifiable property in various genetic backgrounds.