Fig 3.

PgadA::lacZ and PgadE::lacZ fusion activities during growth in minimal and LB media. (A and B) The effects of overproduced GreA and GreB on the gadA and gadE promoter fusion activities were monitored in ppGpp⁰ and ppGpp⁰ dksA strains at 37°C in M9 glucose medium. β-Galactosidase measurements were performed when cultures reached an OD₆₀₀ of ∼0.2. (C and D) The effects of overproduced GreA, GreA*, GreB, DksA, and DksA* on the gadA and gadE promoter fusion activities were monitored in ppGpp⁰ and ppGpp⁰ dksA strains at 37°C in LB. β-Galactosidase measurements were performed before cultures reached an OD₆₀₀ of ∼1.0. When added, IPTG was present at 0.1 mM.