Table 2.

Effect of multicopy greA, greB, and dksA genes on the auxtrophies of ppGpp⁰ strains

Row no.	Strain descriptionb	Plasmid	Growth on M9 platesa
			Without amino acids		With DQ
			− IPTG	+ IPTG	+ IPTG
1	Wild type (± greB)	Vectorc	−	−	−
2		pGreA	−	−	+
3		pGreB	−	−	−
4		pDksA	−	−	+
5	dksA (± greB)	Vector	−	−	+
6		pGreA	−	+	+ (≫)
7		pGreB	−	−	+
8		pDksA	−	−	+ (≫)
9	greA (± greB)	Vector	−	−	−
10		pGreA	−	−	+
11		pGreB	−	−	−
12		pDksA	−	+	+ (≫)
13	dksA greA (± greB)	Vector	−	−	−
14		pGreA	−	+	+ (≫)
15		pGreB	−	−	+
16		pDksA	−	+	+ (≫)

^aGrowth conditions and scoring were as described for Table 1.

^bStrains were as described for Table 1 except that ppGpp⁰ strains were used.

^cThe vector control was pHM1883.