Table 4.
Role of GreA/B and DksA in the M+ mutant suppression of the ppGpp0 phenotype
| M+ mutant strain description | Additional mutation(s) | Growth on the indicated mediuma |
||
|---|---|---|---|---|
| M9 | SMG | AT | ||
| rpoB114(S531F) | None | + | − | − |
| greA | + | − | − | |
| greB | + | − | − | |
| dksA | + | − | − | |
| greA greB | + | − | − | |
| greA dksA | − | − | − | |
| greB dksA | + | − | − | |
| greA greB dksA | − | − | − | |
| rpoB(G534C) | None | + | − | − |
| greA | + | − | − | |
| greB | + | − | − | |
| dksA | + | − | − | |
| greA greB | + | − | − | |
| greA dksA | − | − | − | |
| greB dksA | + | − | − | |
| greA greB dksA | − | − | − | |
| rpoB3443(L533P) | None | + | + | − |
| greA | + | + | − | |
| greB | + | + | − | |
| dksA | + | + | − | |
| greA greB | + | + | − | |
| greA dksA | + | − | − | |
| greB dksA | + | + | − | |
| greA greB dksA | + | − | − | |
| rpoB(L571P) | None | + | + | − |
| greA | + | + | − | |
| greB | + | + | − | |
| dksA | + | + | − | |
| greA greB | + | + | − | |
| greA dksA | + | − | − | |
| greB dksA | + | + | − | |
| greA greB dksA | + | − | − | |
| rpoB3449(A532Δ) | None | + | + | + |
| greA | + | + | + | |
| greB | + | + | + | |
| dksA | + | + | + | |
| greA greB | + | + | + | |
| greA dksA | + | − | + | |
| greB dksA | + | + | + | |
| greA greB dksA | + | − | − | |
| rpoB3370(T563P) | None | + | + | + |
| greA | + | + | + | |
| greB | + | + | + | |
| dksA | + | + | + | |
| greA greB | + | + | + | |
| greA dksA | + | − | − | |
| greB dksA | + | + | + | |
| greA greB dksA | + | − | − | |
a
Growth was monitored by streaking cells on the appropriate medium and scored after 2 days at 37°C. Scoring is otherwise as described for Table 1. M, M9 minimal medium; SMG, 100 mM (each) serine, methionine, and glycine; AT, 18 mM 3-amino-1,2,4-triazole. The ppGpp0 btuB::Tn10 (ppGpp0) control strain does not grow on M, SMG, or AT medium.