Table 1.
Strains and plasmids used and constructed
| Strain or plasmid | Relevant genotype or descriptiona | Resistanceb | Reference or source |
|---|---|---|---|
| Strains | |||
| AB1157Z84 | AB1157 carrying the ftsZ84 mutation | Tc | Gift from David J. Sherratt |
| BL21 A1 | Arabinose-inducible gene expression from T7 promoter | Invitrogen | |
| BTH101 ΔzapA ΔzapB | BTH101 in which the zapA and zapB genes are deleted | This work | |
| C41 | F−ompT hsdSB (rB− mB−) gal dcm | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Invitrogen | |
| MC1000 | Δ(ara-leu) Δlac rpsL150 | 8 | |
| MC1000 ΔzapC | MC1000 zapC::kan | Kan | This work |
| MG1655 | Laboratory collection | ||
| KG22 | C600 lacIqlacZΔM15 | Laboratory collection | |
| KG22Z84 | KG22 carrying the ftsZ84 mutation | Tc | This work |
| KG22Z84 ΔzapB | KG22 carrying the ftsZ84 mutation, in which the zapB gene is deleted | Tc | This work |
| KG22Z84 ΔzapA | KG22 carrying the ftsZ84 mutation, in which the zapA gene is deleted | Tc | This work |
| PS106 | W3110 ftsZ84 | 39 | |
| PS106 ΔzapA | PS106 zapA::kan | Kan | This work |
| PS106 ΔzapB | PS106 zapB::kan | Kan | This work |
| PS223 | W3110 zipA1 | 39 | |
| PS223 ΔzapA | PS223 zapA::kan | Kan | This work |
| PS223 ΔzapB | PS223 zapB::kan | Kan | This work |
| PS236 | W3110 ftsA12 leu::Tn10 | 39 | |
| PS236 ΔzapA | PS236 zapA::kan | Kan | This work |
| PS236 ΔzapB | PS236 zapB::kan | Kan | This work |
| PS413 | W3110 ftsI23 leu::Tn10 | 11 | |
| PS413 ΔzapA | PS413 zapA::kan | Kan | This work |
| PS413 ΔzapB | PS413 zapB::kan | Kan | This work |
| Plasmids | |||
| pBAD33 | Cloning vector containing the PBAD promoter | Cml | 24 |
| pEG3a | PBAD::zapB::gfp | Cml | 16 |
| pEG9 | PBAD::zapB::mCherry | Cml | 19 |
| pEG12 | Plac::ftsZ::gfp | Amp | 19 |
| pEG28 | PoxyTc::zapA | Amp | 19 |
| pEG37 | PT7::zapBΔ10::his6 | Amp | This work |
| pEG43 | Plac::his6::zapA | Amp | This work |
| pEG47 | Plac::his12::zapA | Amp | This work |
| pEG49 | PT7::zapB(S4A L5A)::his6 | Amp | This work |
| pEG58 | Plac::zapA | Amp | This work |
| pEG59 | PBAD::zapA | Cml | This work |
| pEG60 | Ptol::zapB::mCherry | Tc | This work |
| pEG67 | PBAD::zapC | Cml | This work |
| pHis17 | Cloning vector | Amp | 46 |
| pKT25 | T25 fusion vector | Kan | 27 |
| pKT25N | T25 fusion vector | Kan | 27 |
| pKT25N-ZapA-1 | Plac::zapA(aa 1-20)::T25 | Kan | This work |
| pKT25N-ZapA-2 | Plac::zapA(aa 1-47)::T25 | Kan | This work |
| pKT25-ZapA | Plac::T25::zapA | Kan | 19 |
| pKT25-ZapA-3 | Plac::T25::zapA(aa 21-109) | Kan | This work |
| pKT25-ZapA-4 | Plac::T25::zapA(aa 48-109) | Kan | This work |
| pKT25-ZapB | Plac::T25::zapB | Kan | 16 |
| pJB866 | Cloning vector | Tc | 6 |
| pMFV56 | Plac::ftsZ | Kan | 43 |
| pMG25 | Cloning vector | Amp | Laboratory collection |
| pNG53 | PTrc99a::yfp::zapA | Amp | 21 |
| pQE30 | Cloning vector | Amp | Qiagen |
| pUT18C | T18 fusion vector | Amp | 27 |
| pUT18C-ZapA | Plac::T18::zapA | Amp | 19 |
| pUT18C-ZapB-1 | Plac::T18::zapB(aa 11-81) | Amp | This work |
| pUT18C-ZapB-2 | Plac::T18::zapB(aa 21-81) | Amp | This work |
| pUT18C-ZapB-3 | Plac::T18::zapB(aa 31-81) | Amp | This work |
| pUT18C-ZapB-4 | Plac::T18::zapB(aa 41-81) | Amp | This work |
| pUT18C-ZapB-5 | Plac::T18::zapB(aa 1-70) | Amp | This work |
| pUT18C-ZapB-6 | Plac::T18::zapB(aa 1-60) | Amp | This work |
| pUT18C-ZapB-7 | Plac::T18::zapB(aa 1-50) | Amp | This work |
| pUT18C-ZapB-8 | Plac::T18::zapB(aa 1-40) | Amp | This work |
| pUT18C-ZapB(S4A L5A) | Plac::T18::zapB(S4A L5A) | Amp | This work |
| pUT18C-ZapB(E6A V7A) | Plac::T18::zapB(E6A V7A) | Amp | This work |
| pUT18C-ZapB(F8A E9A) | Plac::T18::zapB(F8A E9A) | Amp | This work |
| pUT18C-ZapB(K10A L11A) | Plac::T18::zapB(K10A L11A) | Amp | This work |
| pZapBHis | PT7::zapB::his6 | Amp | 16 |
a
Amino acid truncations are shown following the gene designation. For example, zapA(aa 1-20) represents the zapA gene harboring amino acids 1 to 20.
b
Tc, tetracycline; Kan, kanamycin; Cml, chloramphenicol; Amp, ampicillin.