Fig 3.

Viability and metabolic pattern of the evolved strain ENZ1901 in pure culture starved for P_i. Strains were inoculated (0.1 ml) into 50 ml of P_i-limiting medium (time zero) and incubated further for up to 16 days. The numbers of viable cells in the cultures (A) and the concentrations of glucose (B) and of acetate (C) in the spent medium were determined. Evolved strain ENZ1901 (Km^r, ENZ2000) (▴ and ▾), ancestral strain ENZ535 (Tc^r, ENZ1797) (▵ and ▿), and ENZ535 carrying rpoS::Tn10 (ENZ985) (○) and ΔrpoS (ENZ2020) (□) were used.