Table 1.
Bacterial strains and constructs
| Strain or plasmid | Relevant characteristicsa | Reference(s) or source |
|---|---|---|
| Strains | ||
| E. coli DH5a | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169recAlendAIhsdR17 (rK− mK+) supE44 λ−thi-1gyrA96relAl | |
| P. syringae pv. maculicola ES4326 | Wild type; Smr | 16 |
| P. syringae pv. maculicola YM7930 | Wild type | MAFF |
| P. syringae pv. thea K93001 | Wild type | MAFF |
| Plasmids | ||
| pCKTR | pBluescript derivative with recA internal fragment; Kmr | 28 |
| pCKTR-E | pCKTR derivative containing E622; Kmr | This study |
| pCKTR-Ea | pCKTR-E derivative with E622::bla; Kmr Apr | This study |
| pLAFR | Broad host range cosmid; Tcr | 15, 70 |
| pLAFR-Ea | pLAFR derivative containing transposed E622::Apr | This study |
| pUCP20Tk | Kmr derivative of pUCP20T (73) | This study |
| pUCP20Tk-Ea | pUCP20Tk containing pLAFR-Ea fragment; Apr Kmr | This study |
a
Abbreviations: Sm, streptomycin; Tc, tetracycline; Ap, ampicillin; Km, kanamycin.