Table 1.
Plasmids and strains used in this study
| Strain or plasmid | Genotypea | Reference or source |
|---|---|---|
| Strains | ||
| P. putida | ||
| KT2440 | Cmr | 11 |
| phhR strain | Kmr | 19 |
| crp strain | Cmr Kmrcrp::mini-Tn5 | 8, 9 |
| cya strain | Cmr Kmrcya::mini-Tn5 | 8, 9 |
| E. coli | ||
| BL21(DE3) | F−ompIhsdSB (rB− mB−) galdemmet | Novagen |
| DH5αF′ | F′ hsdR17recA1gyrA | 49 |
| Plasmids | ||
| pMP220 | ′lacZ IncP Tcr | 45 |
| pMCA | PphhA::lacZ Tcr | 19 |
| pET28a (+) | Kmr protein expression vector | Novagen |
| pBend2 | Apr circular permutation vector | 24 |
| pCR2.1 | Apr Kmr | Invitrogen |
| pMCPhhR | KmrphhR | 19 |
| pCRP1 | Apr Kmrcrp | This study |
| pCRP2 | Apr Kmr Crp expression | This study |
| pCRP3 | Apr Kmr PPhhAB | This study |
| pMCCrp | Kmrcrp | This study |
| pCRP3 | Apr | This study |
| pGEMT-phhA-v | Apr, mutant, phhA variant, deleted of the Crp binding site | This study |
| pPhhRBend | Apr | This study |
a
Apr, Cmr, Kmr, and Tcr, resistance to ampicillin, chloramphenicol, kanamycin, and tetracycline, respectively.