Fig 2.

The C. jejuni strain contains a single enzyme with catalase activity, and the ΔCj1386 mutant exhibits a reduced catalase activity. All bacterial strains (C. jejuni NCTC11168 and ΔkatA and ΔCj1386 mutants) were grown in MEMα to mid-log phase (OD₆₀₀ of 0.2) under microaerophilic conditions at 37°C prior to cell extract preparation. When required, wild-type C. jejuni was exposed to 1 mM H₂O₂ for 10 min prior to total protein isolation. Fifty micrograms of whole-cell extract was loaded onto an 8% nondenaturing gel run at 25 mA at 4°C and assayed for catalase activity. BLC, bovine liver catalase (Sigma-Aldrich).