Fig 3.

Cj1386 enhances KatA catalase activity. (A) Catalase activities of cytoplasmic and periplasmic cellular protein fractions for wild-type C. jejuni and ΔCj1386, ΔCj1386+Cj1386, ΔkatA, and ΔkatA+katA strains are expressed as μmol of hydrogen peroxide decomposed per minute per mg of protein. Error bars indicate standard errors of the means (n = 4). An asterisk indicates a P value of <0.05 using the Student t test. (B) Cell fractionation control assay using Western blot analysis. Five micrograms of wild-type C. jejuni and ΔCj1386 mutant cytoplasmic and periplasmic protein extracts were loaded into each well and subsequently assessed for cytoplasmic protein contamination by immunoblotting using an anti-PerR antibody.