Fig 4.

KatA expression is not affected in the ΔCj1386 mutant, as shown by Western blot analysis of KatA protein content in wild-type C. jejuni; ΔCj1386, ΔkatA, and ΔkatA+katA mutants; and affinity-purified C. jejuni KatA. (A) Five micrograms of protein lysate or 100 ng of purified protein was loaded into each lane followed by immunoblotting using anti-KatA or anti-Fur antiserum. (Top) KatA protein level as detected by anti-KatA antiserum. (Bottom) Loading control of total protein content detected by anti-Fur antiserum. (B) Quantification of KatA protein level in wild-type C. jejuni, ΔCj1386, ΔkatA, and ΔkatA+katA strains. Relative intensity of KatA was determined by quantifying each band from the immunoblot and standardizing it against WT KatA. Error bars represent the standard errors of 3 biological replicates.