Table 2.
Primers used in this study
| Primer name | Primer sequence (5′–3′)a |
|---|---|
| Cj1386-sense | CGGTACCCGGGGATCCAAGGCGTAGCACA |
| AAGATATGC | |
| Cj1386-antisense | CGACTCTAGAGGATCCAAAAAGAAGAAATTGCTGAAAAGC |
| Cj1386-SE-inverse | GAACTAAAGGGCGCATTCTTATGAAAGCGCTAAAATGC |
| Cj1386-AS-inverse | GAACACCGCCGAGCAGCAAGCATAAGCAAGCTATCG |
| katA + Cj1386-SE | CGGTACCCGGGGATCCCGATTTTGGAAACATTATAGCTGA |
| katA + Cj1386-AS | CGACTCTAGAGGATCCTAAAAGGGGCGGTTCCTATC |
| katA + Cj1386-SE-inv | GAACTAAAGGGCGCAGGCGATAGCTTGCTTATGCT |
| katA + Cj1386-AS-inv | GAACACCGCCGAGCACGGATGAAGAATGTCGGAGT |
| Cj1386-SE-comp | GATTTAGATGTCTAGCGCTTCTATGGAAGGAGTTGA |
| Cj1386-AS-comp | GGGGAAGCTTTCTAGAAAAAGAAGAAATTGCTGAAAAGC |
| katA + Cj1386-SE-comp | GATTTAGATGTCTAGTTACGTGCATCCCAGTGTTC |
| katA + Cj1386-AS-comp | GGGGAAGCTTTCTAGTAAAAGGGGCGGTTCCTATC |
| Cat-SE | TGCTCGGCGGTGTTCCTTTCCAAG |
| Cat-AS | TGCGCCCTTTAGTTCCTAAAGGGT |
| AR56-AS | CATCCTCTTCGTCTTGGTAGC |
| ak233-SE | GCAAGAGTTTTGCTTATGTTAGCAG |
| ak234-SE | GAAATGGGCAGAGTGTATTCTCCG |
| ak235-SE | GTGCGGATAATGTTGTTTCTG |
| katA-RT-SE | AAGTGGAGCTTATGGCGAAA |
| katA-RT-AS | ACTTCGCTTTTTGCACGATT |
| katA-RTint-SE | AGCTGCCGAGCTTATAGCAA |
| Cj1386-RT-SE | GAGCTTTGCAAAATGGCTTT |
| Cj1386-RT-AS | CCACATCTTTGCGTCCAAAC |
| Cj1386-RTint-AS | TAATGGGGTTTGTCCACGAT |
| Cj1384c-RT-SE | TGGGATGCACGTAATGAAAA |
| Cj1387c-RT-AS | TTGATTTTCCAACAAGAGCCTTA |
| katA-SE-qPCR | GCGATGTGAGAGGTTTTGCT |
| katA-AS-qPCR | CAAAAATCCCAAGCAGCATT |
| Cj1386-SE-qPCR | GGCGATAGCTTGCTTATGCT |
| Cj1386-AS-qPCR | TAATGGGGTTTGTCCACGAT |
| metC_SE | CTAAACTTATTCATTGTGGCAGAGG |
| metC_AS | CTCTGTATTTTTCCAAGTTGCGTG |
| KatA_NcoI | GCCATGGCTATGAAAAAATTGACTAACGA |
| KatA_NotI | GCGGCCGCTTAGTTTGCCACCAAAAGTGG |
a
Restriction sites are shown in bold.