Table 3.
Sensitivity of wild-type C. jejuni, ΔkatA and ΔCj1386 mutants, and corresponding complemented strains to three oxidantsa
| Strain | Diam (mm) of zone of inhibition after exposure to oxidant: | ||
|---|---|---|---|
| H2O2 | CHP | MND | |
| C. jejuni NCTC11168 | 19.70 ± 0.38 | 22.90 ± 0.52 | 34.8 ± 0.79 |
| ΔkatA mutant | 25.30 ± 0.07* | 24.58 ± 0.19 | 29.85 ± 1.39 |
| ΔkatA+ katA strain | 10.83 ± 1.24 | 23.63 ± 0.47 | 31.00 ± 1.20 |
| ΔCj1386 mutant | 25.21 ± 0.28* | 25.67 ± 0.76 | 35.04 ± 1.36 |
| ΔCj1386 + Cj1386 strain | 19.90 ± 0.57 | 24.20 ± 0.51 | 37.30 ± 0.76 |
| ΔkatAΔCj1386 mutant | 25.80 ± 0.39* | 22.90 ± 0.71 | 35.30 ± 1.12 |
| ΔkatAΔCj1386 + (katA + Cj1386) strain | 10.70 ± 0.85 | 23.90 ± 0.40 | 37.50 ± 0.60 |
a
The diameter of the zone of inhibition is represented as the mean clear zone ± standard error for each strain (in mm) after exposure to 10 μl of 3% H2O2 (hydrogen peroxide), 3% cumene hydroperoxide (CHP), or 90 mM menadione (MND) bisulfite. Each experiment was repeated in quadruplicate. Values were considered significant (*) at P < 0.05 using ANOVA statistical analysis.