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Screening of P. gingivalis substrates using patient-derived paper points. A total of 72 patient-derived paper points were incubated with 16 μM each FRET substrate. Fluorescence was measured for 90 min at 37°C. Enzyme activity was defined in RF/min. The cutoff of the assay was estimated at an RF/min value of 5. Results are expressed as mean ± standard error of the mean. 107 CFU/ml, n = 6; 106 CFU/ml, n = 6; 105 CFU/ml, n = 5; 104 CFU/ml, n = 3; <10 CFU/ml, n = 52.
