Table 1.

Performance characteristics of the PCR-Luminex assay to detect bacterial enteropathogens

Pathogen	Strain	Second-order polynomial regression		Limit of detectiona (CFU/g stool)	Precisionb (% CV)
		Reportable range (copies/PCR)	R2		Within run	Between runs
Aeromonas hydrophila	Clinical isolate	102–107	0.98	104	11.12	15.82
Campylobacter jejuni	ATCC 33291	102–107	0.96	103	9.58	16.27
Campylobacter coli	ATCC 43473	103–107	0.91	105	11.10	18.63
Salmonella Enteritidis	SARB no. 16	102–107	0.95	103	7.02	12.09
Shigella sonnei	Clinical isolate	102–107	0.92	103	10.51	15.30
Vibrio cholerae	Clinical isolate	102–107	0.97	104	6.20	14.78
Vibrio parahaemolyticus	ATCC 17802	103–107	0.89	105	11.77	19.73
Yersinia enterocolitica	ATCC 23715	102–107	0.98	104	4.72	6.07

^aLimit of detection was determined with control fecal samples spiked with the lowest amount of bacterial culture that could be detected in 10/10 samples.

^bWithin-run precision and between-run (over 5 days) precision were both tested on 10 samples.