Table 3.
Testing of Luminex PCR/conventional test-discrepant samples, with qPCR targeting a second gene
| qPCR targeting a second gene | No. of samples |
% correlation between secondary qPCR and Luminex | |
|---|---|---|---|
| Luminex PCR positive/conventional test negative | Luminex PCR negative/conventional test positive | ||
| Aeromonas 16S qPCR positive | 10 | 0 | 93 |
| Aeromonas 16S qPCR negative | 1 | 4 | 93 |
| Campylobacter 16S qPCR positive | 11 | 4a | 76 |
| Campylobacter 16S qPCR negative | 0 | 2 | 76 |
| Salmonella ompC qPCR positive | 9 | 0 | 100 |
| Salmonella ompC qPCR negative | 0 | 1 | 100 |
| Shigella virA qPCR positive | 7 | 0 | 83 |
| Shigella virA qPCR negative | 2 | 3 | 83 |
| Vibrio hlyA/tlh qPCR positive | 6 | 0 | 100 |
| Vibrio hlyA/tlh qPCR negative | 0 | 2 | 100 |
a
These four Campylobacter 16S products were sequenced, and three were C. upsaliensis and one was C. hyointestinalis.