. 2012 Jan;50(1):91–97. doi: 10.1128/JCM.05612-11

Table 4.

Comparison of LAMP and qPCR assays in human stool specimens spiked with 10³ and 10⁴ CFU/0.5 g of STEC O157 strain EDL933 based on two independent repeats

Cell level (no. of CFU/0.5 g)	Target gene	LAMP T_t (min) after enrichment of^b:				qPCR C_T (cycles) after enrichment of^b:
Cell level (no. of CFU/0.5 g)	Target gene	0 h	4 h	6 h	8 h	0 h	4 h	6 h	8 h
10³	stx₁	NA^c	32.0 ± 2.6^A	20.9 ± 1.5^B	17.3 ± 1.6^B	NA	37.9^a	34.1 ± 1.5^A	29.1 ± 1.4^A
	stx₂	NA	33.9 ± 2.3^A	23.9 ± 1.1^B	21.0 ± 1.3^B	NA	NA	34.3 ± 1.5^A	29.4 ± 1.5^A
	eae	NA	27.8 ± 2.1^A	19.0 ± 2.0^B	15.9 ± 1.8^B	NA	37.2^a	32.3 ± 1.4^A	26.6 ± 1.5^A
10⁴	stx₁	NA	22.9 ± 0.8^A	18.9 ± 0.4^B	18.2 ± 0.5^B	NA	34.5 ± 1.4^A	31.3 ± 1.5^A	30.2 ± 1.4^A
	stx₂	NA	26.6 ± 1.3^A	23.2 ± 0.5^AB	21.5 ± 1.3^B	NA	34.5 ± 1.2^A	31.2 ± 1.4^AB	30.3 ± 1.3^B
	eae	26.9^a	19.8 ± 3.0^A	17.5 ± 1.4^A	17.0 ± 1.1^A	NA	33.5 ± 1.8^A	29.7 ± 1.5^A	28.8 ± 1.1^A

Only one repeat generated a positive result.

In each row within LAMP or qPCR, T_t or C_T values followed by different uppercase letters are statistically significant (P < 0.05).

NA, not available.