Figure 1.

Inhibition of myeloma cell proliferation. (a) The dose-dependent effect of compounds on the proliferation of myeloma cell lines was evaluated by the modified MTT assay. Cells were incubated with serial dilutions of compounds for 72 h. Data represent the means±s.d. of three independent experiments, and four replicate experiments. (b) Time-dependent inhibition of proliferation of IM-9 cells. Cells were incubated with AV-65 for 6, 12, 24 and 48 h. Data represent the means±s.d. of three independent experiments, and four replicate experiments. (c) Effect of compounds on CD138-positive myeloma cells and CD138-positive normal plasma cells. CD138-positive myeloma cells and CD138-normal plasma cells were purified from BM mononuclear cells obtained from myeloma patients and healthy volunteers, respectively. Data represent the mean of four replicates for each concentration.