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. 2011 Nov 4;1(11):e43. doi: 10.1038/bcj.2011.41

Figure 3.

Figure 3

Inhibition of β-catenin-TCF signaling by AV-65 treatment. (a) Inhibition of TCF transcriptional activity in HCT-15 colorectal cancer cells. Cells were co-transfected with a TCF/LEF firefly luciferase reporter, and a CMV-driven Renilla luciferase reporter. To normalize transfection efficiency, cells were incubated with AV-65 for 14 h, and then luciferase activity was measured. Data represent the mean±s.d. of three independent experiments. The relative TCF transcription activity represents the difference between the relative luciferase units of treated versus non-treated cells. (b) Decreased expression of TCF downstream targets in IM-9 cells. Cells were incubated with serial dilutions of AV-65 and then harvested at 24 h. Harvested cells were lysed in radioimmunoprecipitation assay buffer. Cell extracts (20 μg of protein) was analyzed by western blot using the indicated Abs. Cyclin D1 and c-myc were not expressed in IM-9 and U266 cells, respectively.