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. 2011 Nov 4;1(11):e38. doi: 10.1038/bcj.2011.37

Figure 2.

Figure 2

Western blotting analysis of lysate from leukemia cell lines. (After 48 h of addition or no addition of TNF-α (5 ng/ml)). All the band were quantified and normalized by β-actin. Normalized densities of SEM cells without TNF-α were standardized as 1.00. Upregulation of S100A6 expression and downregulation of acetyl-p53/p53 expression ratio, cleaved caspase 3 and cleaved caspase 8 expression only in MLL–AFF1-positive ALL cell lines in the presence of TNF-α (5 ng/ml), although there were no significant differences between the two groups in the absence of TNF-α. There were no differences of TNF receptor 1 (TNFR1) expression in the absence or presence of TNF-α. Densitometry measurements were standardized by those of SEM without TNF-α.