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. 2011 Nov 4;1(11):e38. doi: 10.1038/bcj.2011.37

Figure 3.

Figure 3

Effect of S100A6 siRNA or MLL–AFF1 siRNA on MLL–AFF1-positive cell lines. (a) Real-time quantitative PCR analysis of S100A6 mRNA and MLL–AFF1 mRNA under S100A6 siRNA or MLL–AFF1 siRNA or control siRNA in the presence of TNF-α (5 ng/ml) of MLL–AFF1-positive cell lines (SEM and RS4;11). S100A6 mRNA expression was significantly inhibited by both MLL–AFF1 siRNA and S100A6 siRNA in comparison with control siRNA. MLL–AFF1 mRNA expression was significantly inhibited by MLL–AFF1 siRNA in comparison with control siRNA, but MLL–AFF1 expression was not significantly inhibited by S100A6 siRNA. (b) Apoptotic rate by S100A6 siRNA or MLL–AFF1 siRNA or control siRNA in the presence of TNF-α (5 ng/ml) of MLL–AFF1-positive cell lines (SEM and RS4;11). The apoptotic rate of MLL–AFF1-positive ALL cell lines treated with siRNA against MLL–AFF1 in the presence of TNF-α (5 ng/ml) was significantly higher than those treated with control siRNA. The apoptotic rate of MLL–AFF1-positive ALL cell lines treated with siRNA against S100A6 in the presence of TNF-α was significantly higher than those treated with mismatch control siRNA.