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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Methods Mol Biol. 2012;796:217–231. doi: 10.1007/978-1-61779-334-9_12

Figure 3. Analysis of the disproportionation of subunits between HsPBGS oligomers.

Figure 3

A) Native PAGE analysis of WT+F12L HsPBGS oligomers before and after dialysis under turnover conditions demonstrates that substrate turnover facilitates oligomeric interconversion. Following dialysis to yield mixed hetero-oligomers, the hetero-hexamers were resolved from the hetero-octamers by anion exchange chromatography. B) Mass spectroscopy focusing on the N-terminal tryptic peptides of HsPBGS oligomers before and after substrate turnover conditions demonstrates subunit exchange during oligomeric interconversion.