FIGURE 2.

Identification of the PABP binding site of MKRN1-short. A, shown is a schematic representation of recombinant EGFP-MKRN1-short fusion proteins (encoded by M1-M8) that were transiently expressed in HEK-293 cells together with FLAG-PABP. B–F, protein extracts were subjected to immunoprecipitation with anti-FLAG-agarose followed by SDS-PAGE and Western blot (WB) analyses using anti-GFP antibodies for detection of EGFP-MKRN1 deletion mutants (B–D) or anti-PABP antibodies (E and F). B and E: inputs; C and F, eluted immunoprecipitates; D, supernatant after immunoprecipitation. The positions of molecular size marker proteins (in kDa) are indicated on the right. E, MTZF, Makorin-type zinc-finger; RFΔCC, truncated RING finger domain.