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. 2011 Oct 31;287(2):1458–1467. doi: 10.1074/jbc.M111.253658

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Restoration of binding capacity and epitope identification on human CXCL10 and CXCL9. Specific binding of E7, J9, P8, F13, C1, and J5 dual-specific scFvs to a panel of CXCL10 (A and B) and CXCL9 (C and D) mutants was assessed in an ELISA. The indicated NusA-fusion chemokines were captured and then incubated with scFvs. Coating was controlled using specific anti NusA mAb, and NusA protein was also added to the assay as negative control (data not shown). Results are expressed as mean ± S.D. of duplicates. hum10, human CXCL10; rab10, rabbit CXCL10; hum9, human CXCL9; cyn9, CXCL9.