FIGURE 2.
ITC data. Varied doses of TrCel12A, TrCel5A, and TrCel7B added to 8 g/liter RAC at 30 °C, pH 5. 0 are shown. The raw data (μJ/s) has been converted to nm/s using the ΔappH determined from Fig. 1, and the distinctive burst of all three EGs is evident. The enzyme was added under vigorous (400 rpm) stirring at time = 0. The stirring was stopped after the injection was complete at time = 60 s, and the hydrolysis was monitored for 15 min. For a more detailed account see the supplemental material Fig. S1.