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Validation of CUGBP1 target transcripts by RT-PCR. RNA-IP was performed using cytoplasmic lysates from unstimulated (−) and stimulated (+) T cells and antibodies targeting PABP (α-PABP), HA (α-HA), and CUGBP1 (α-CUGBP1). The levels of Topoisomerase I (TOP1), JunD proto-oncogene (JunD), ubiquitin-protein ligase E3A (UB3EA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) transcripts were measured in the immunoprecipitated material by RT-PCR.
