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. 2011 Nov 28;287(2):1538–1544. doi: 10.1074/jbc.M111.302463

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Full-length CwpV retains its aggregation-promoting properties. A, aggregation of C. difficile from liquid culture (starting A_{600 nm} of 4) observed in suspensions of C. difficile ΔcwpV (pCwpV (WT)), ΔcwpV (pCwpV_K410G/D411G), ΔcwpV (pCwpV_T413S), ΔcwpV (pCwpV_T413C), ΔcwpV (pCwpV_T413A), and ΔcwpV (pCwpV_T413V) but not C. difficile 630. B, colony morphology caused by CwpV overexpression in C. difficile. All strains overexpressing CwpV show a smaller, smoother edged morphology compared with the larger, ruffled edged colonies of C. difficile 630 (WT).