FIGURE 2.
MOV10 packaging into HIV-1 requires the NC basic linker. A, schematic illustrates p55Gag and NC for creating Gag-CFP fusion constructs. The number indicates the C-terminal AA residue expressed, with the first Gag residue considered 1. B, MOV10 or A3G was expressed with indicated Gag-CFP fusion proteins in 293T cells. Virions were purified through a 20% sucrose cushion via ultracentrifugation. Cellular and virion-associated proteins were determined by Western blotting with the indicated antibodies.