FIGURE 3.

HIV-1 Gag binds to the N-terminal AA 261–305 region of MOV10. A, schematic illustrates MOV10 N-terminal or C-terminal deletion mutations. Numbers indicate AA positions. The locations of the putative CH domain (see Fig. 6) and seven helicase motifs are indicated. The border of the putative CH domain, the first residue of the helicase motif I, and the last residue of the helicase motif VI are numbered. B, Gag binding domain of MOV10 was mapped by immunoprecipitation (IP). The indicated MOV10 deletion mutants were expressed with HA-tagged codon-optimized HIV-1 HXB2 Gag in 293T cells. Proteins were pulled down by anti-HA antibody and analyzed by Western blotting.