FIGURE 5.

All but one of seven helicase motifs are also required for MOV10 virion packaging. A, anti-HIV-1 activity and virion incorporation of MOV10 helicase mutants. The indicated MOV10 mutants were expressed with VSV-G pseudotyped pNL-Luc reporter viruses in 293T cells, and viral infectivity was analyzed in CEM-SS cells. Viral infectivity is presented as relative value, with the infectivity of viruses produced in the presence of a control vector as 100%. The S.E. were calculated from three independent experiments. In addition, virions were purified by ultracentrifugation, and cellular and virion proteins were analyzed by Western blotting. B, interaction of MOV10 helicase mutants with HIV-1 Gag, as determined by the same as in Fig. 3B.