FIGURE 1.

Nucleotide and deduced amino acid sequences of PfI3. A, amino acids are numbered to the right of the sequence. The sequences of 5′- and 3′-untranslated regions were obtained using primers derived from genomic DNA followed by PCR on cDNA and by performing 3′-rapid amplification of cDNA ends, respectively. B, analysis of amino acid sequence of PfI3. PfI3 was aligned with the mammalian (Inhibitor-3) and yeast I3 (Ypi1) homologs using the Megalign program (DNASTAR). The identical residues are shown in gray. The box contains the KVVRW sequence which fits with the consensus sequence (K/R)X_0–1(V/I)p(F/W), known as the RVXF motif and required for binding with PP1.