FIGURE 9.

Expression of PfI3 gene products by transfected P. falciparum. A, schematic representation of the pARL2-PfI3-GFP used for episomal expression of PfI3. The construct contains the complete open reading frame of PfI3 in fusion with GFP. B, plasmid rescue experiments showing the presence of pARL2-PfI3-GFP constructs in transfected parasite culture. C, immunoblot analysis of pARL2-PfI3-GFP transfected P. falciparum. Proteins extracted from wild type parasites (lane 1) or from transfected parasites (lane 2) were subjected to Western blotting and probed with anti-GFP antibodies. D, expression and localization of PfI3-GFP throughout the erythrocytic cell cycle of P. falciparum. Parasites were transfected as described under “Experimental Procedures,” and live transfectants were analyzed by fluorescence microscopy.