FIGURE 6.

Real-time analysis of β-catenin intracellular distribution in response to CaSR stimulation. A, NCMiCaR cells expressing or not the CaSR were transfected with a construct encoding RFP-β-catenin or RFP as indicated under “Experimental Procedures.” The intracellular distribution of RFP-β-catenin and RFP in response to 5 mm Ca²⁺-mediated CaSR stimulation was examined in real time with an epifluorescence microscope. Quantification was performed as described under “Experimental Procedures.” The values represent the MI ± S.E., and they were compared by unpaired Student's t test (*, p < 0.01). Scale bar, 10 μm. B, CaSR-mediated β-catenin transcriptional activity regulation. NCMiCaSR cells not expressing (Control) or expressing the CaSR were challenged with 5 mm Ca²⁺ or 100 nm R-568 and 1.4 mm Ca²⁺, and the luciferase activity was determined as described under “Experimental Procedures.” The luciferase activity values are the result of three independent experiments ± S.E., and they were compared using unpaired Student's t test (*p < 0.01).