FIGURE 2.

mi264 embryos have a mutation in Connexin 39.9. A, meiotic mapping placed the mi264 locus between myotubularin related protein 2 and zgc:66417 in chromosome 5. B, in mutants, a missense mutation (E103K) was found in an intracellular loop between membrane domains 2 and 3. C, protein alignment of the GJA3 group of connexins in vertebrates. Shaded residues indicate conserved amino acids. The gray boxes represent the membrane domains. Note that the Glu-103 residue, which is mutated in cx39.9 mutants, is completely conserved among vertebrates.