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. 2011 Nov 23;287(2):1510–1519. doi: 10.1074/jbc.M111.285528

FIGURE 2.

FIGURE 2.

Defining disulfide linkages in the C-terminal segment of IGFBP-5. Results demonstrate that peptides P9-P10-P11 and P7-P8 are each linked by disulfide bonds. See supplemental Table S1 for additional information. A and B, analysis of P9-P10-P11. A, ETD-MS2 of precursor ion, m/z 604.8 (+7), corresponding to P9-P10-P11, and containing cysteine residues 210, 221, 223, and 243. B, CID-MS3 of precursor ion, m/z 1128.5 (+3), of disulfide-linked peptides P10 and P11. The y ions marked with an asterisk are from P10; other y ions are from P11. C and D, analysis of P7-P8. C, CID-MS2 of precursor ion m/z 521.5 (+4) of disulfide-linked peptides containing cysteine residues 172 and 199. The y ions are from P8. D, ETD-MS2 of precursor ion m/z 521.5 (+4). In A and D [M] denotes the parent mass. Additional y ions were identified in B and C, but have been removed from the graphs for clarity.