FIGURE 3.

CB₂ cannabinoid receptor activation promotes G₁/S phase progression of neural progenitors through activation of the PI3K/Akt/mTORC1 axis. A, HiB5 cells were treated with HU-308 (HU; 50 nm) alone or in the presence of SR144528 (SR, 2 μm), and cell cycle analysis was performed after DNA content quantification by flow cytometry analysis. The relative fraction of cells in G₀/G₁ and S phase is shown. B and C, cell cycle analysis was performed after HU-308 administration alone or in the presence of rapamycin (Rapa; 50 nm). The fraction of cells in G₀/G₁ and S phase and a representative DNA histogram of each condition are shown. *, p < 0.05; **, p < 0.01 versus vehicle (V)-treated cells; #, p < 0.05; ##, p < 0.01 versus HU-308-treated cells.