FIGURE 2.

A Cytoscape network view of all NMPDR subsystem annotated open reading frames from S. pyogenes. The left part represents an outline of the network topology of the S. pyogenes (strain SF370) proteome displayed using Cytoscape. Rectangles indicate proteins and lines connecting the dots represent functional categories using NMPDR subsystem classification. Sets of rectangles grouped into clusters represent one functional category and rectangles connected with more than one line represent a protein present in several functional categories. The rectangle color scheme indicates changes in protein abundance as a function of increasing plasma concentration shown in Fig. 1. Proteins that show constant abundance are colored green, proteins with increasing abundance are blue, decreasing proteins are red, non-quantified proteins dark gray, and non-identified proteins are represented as light gray rectangles. In this view all hypothetical proteins and proteins without subsystem annotations are outlined at the bottom as unconnected rectangles. Several functional protein categories were significantly enriched in the clusters: A, ribosomal proteins (SSU); B, ribosomal proteins (LSU); C, de novo pyrimidine synthesis; D, choline and betaine uptake and betaine biosynthesis; E, control of cell elongation; F, ribonucleotide reduction; G, fatty acid biosynthesis, FASII.