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. 2011 Nov 17;287(2):1022–1031. doi: 10.1074/jbc.M111.284067

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Interactions of Ala-291 in Af1503 with transient amino acids in serine signaling. Combination of A291I with Ile mutants at transient positions Thr-281, Ser-318, and Ser-325 results in serine regulation of chimeras (the mutated amino acids are inverted in the sequence above). Basal activities were set at 100%: filled squares, T281I/A291I; open squares, A291I/S318I; circles, A291I/S325I. Western blots of 0.5 μg membrane preparation of each construct are shown at the right with the respective symbol on top.