FIGURE 2.

Recognition of 30 peptides sampled at random from a large peptide set (motif, RQWGPDP{A/C/D/F/H/I/K/L/M/N/P/R/S/V/Y}{A/C/G/H/I/K/L/M/N/P/Q/R/S/T/V}A; total set size = 225). 6 × 10⁴ C1R-A2 cells were pulsed with peptides at various concentrations. After 2 h, 3 × 10⁴ 1E6 CD8⁺ T cells were added and incubated overnight. Supernatant was harvested and assayed for MIP1β. Each panel displays titrations of five different peptides relative to index. A, titrations of peptides with the highest functional sensitivities. F, titrations of peptides with the lowest functional sensitivities. Error bars, S.D. from the mean of two replicates. pEC₅₀ values for each peptide are displayed in supplemental Table S3.