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Effects of Hed1 mutations on DSB targeting of Rad54. JKM179 cells that express Myc-tagged Rad54 (36) were transformed with the pTB326 vector (ADH promoter), with pTB326-FLAG-HED1, or pTB326-FLAG-hed1 mutants as indicated. The DSB recruitment of Hed1 and Rad54 to MAT Z was evaluated at 4 h after the induction of an HO break. The MAT Z signal was quantified as described (36). Quantifications were based on three independent experiments, with error bars representing S.E.
