FIGURE 5.

PDE11 is restricted to brain and predominantly expressed in hippocampus. A newly generated antiserum against the PDE11 GAF domains was used to identify PDE11 expression in organs (A) and brain regions (B) of mice in Western blots. Human PDE11A4 (105 kDa) served as a positive control. Preabsorption of the antiserum with purified GAF domains (+ antigen; 4 μg × ml⁻¹) was performed to check signal specificity and abolished PDE11A4 signals in hippocampus, medulla, and eminentia mediana. No indication was found that the 80-kDa signal in aorta and lung is related to PDE11 (see “Results”). Shown is one representative blot of three. Larger sections of Western blots are shown in supplemental Figs. 4 and 5.