TABLE 3.
ITC analysis of AdoCys and pCho binding
Titrations were performed at 20 °C and data fit to a single-site binding model as described under “Experimental Procedures.”
| Protein-Ligand | Kd | ΔG | ΔH | −TΔS |
|---|---|---|---|---|
| μm | kcal mol−1 | kcal mol−1 | kcal mol−1 | |
| PfPMT-AdoCys | 7.9 ± 0.8 | −6.8 ± 0.7 | −11.0 ± 0.3 | 4.2 |
| Y19F-AdoCys | 12.0 ± 1.8 | −6.6 ± 1.0 | −5.3 ± 0.2 | −1.3 |
| H132A-AdoCys | 12.2 ± 2.4 | −6.6 ± 1.3 | −1.6 ± 0.1 | −5.0 |
| PfPMT-pCho | 23.4 ± 1.6 | −6.2 ± 0.4 | −22.3 ± 0.7 | 16.1 |
| Y19F-pCho | 20.2 ± 6.8 | −6.3 ± 2.1 | −1.9 ± 0.2 | −4.4 |
| H132A-pCho | 26.0 ± 11.3 | −6.2 ± 2.7 | −1.6 ± 0.2 | −4.5 |