FIGURE 7.
Current density of IBa through WT and 43* spliced variant channels and gating charge. A and B, peak of tail currents measured at the end of short depolarizing pulses evoked at different potentials were normalized against the membrane capacitance (Cm) of the recorded cell to obtain the current density. The number of cells recorded are shown in parentheses. C, gating charge was calculated by taking the integral of the current transient (gray areas, inset), at the onset, of the depolarizing pulse to the reversal potential of the ionic current. Shown here are representative gating and ionic tail current traces evoked at reversal potential. D, average gating charge for CaV1.4 WT (n = 14) and CaV1.4 43* (n = 21) normalized against membrane capacitance. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (compared with WT; unpaired t test).