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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Osteoarthritis Cartilage. 2011 Sep 16;19(12):1449–1457. doi: 10.1016/j.joca.2011.09.004

Fig. 5.

Fig. 5

In vitro reduction of the IL-1β transcript influences transcript expression of the catabolic mediators MMP13 (A), MMP2 (B), IFN-γ (C), and iNOS (D). In general, transduction with the targeting knockdown vector (TV) significantly reduced the genes of interest in all media conditions relative to the mock-transduced, no vector control (C) and non-targeting vector control (NTC). Within each media condition (control, LPS, or IL-1β), fold differences and subsequent percent gene expression levels (mean ± 95% confidence intervals) for TV and NTC relative to C were calculated using the comparative CT (2−ΔΔCT) method. Data (triplicate assay values for triplicate culture samples) were analyzed using one-way ANOVA for comparison among groups (N, NTC, and TV) followed by pairwise comparisons using Tukey 95% confidence intervals; significant differences are indicated.