. Author manuscript; available in PMC: 2012 Dec 1.

Published in final edited form as: Osteoarthritis Cartilage. 2011 Sep 16;19(12):1449–1457. doi: 10.1016/j.joca.2011.09.004

Table I.

Guinea pig specific primers used for real-time RT-PCR

Transcript of interest	Primer sequences (5′–3′)
GAPDH^*	F: GTATCGTGGAAGGACTCATGACC
GAPDH^*	R: GTTGAAGTCACAGGACACAACCT
eGFP^*	F: CATGATATAGACGTTGTGGCTGTTG
eGFP^*	R: AAGCTGACCCTGAAGTTCATCTGC
IL-1b^*	F: ACGCCTGGTGTTGTCTGAC
IL-1b^*	R: GGGAACTGAGCGGATTC
18S ribosomal RNA^†	F: TGCATGGCCGTTCTTAGTTG
18S ribosomal RNA^†	R: AGTTAGCATGCCAGAGTCTCGTT
IL-2^‡	F: CTTAAGCTCTCCAAAGCA
IL-2^‡	R: CCATCTCTTCAGAAATTCCAC
IL-4^§	F: CATCGGCATTTTGAACGAGGTCA
IL-4^§	R: CTATCGATGAATCCAGGCATCG
IL-6^§	F: CAGCCCTGAGAAAGGAGACAT
IL-6^§	R: AATCTGAGGTGCCCATGCTAC
IL-8^†	F: GGCAGCCTTCCTGCTCTCT
IL-8^†	R: CAGCTCCGAGACCAACTTTGT
IL-10^‡	F: GGCACGAACACCCAGTCTGA
IL-10^‡	R: TCACCTGCTCCACTGCCTTG
IL-12^‡	F: TCTGAGCCGGTCACAACTGC
IL-12^‡	R: AGGCGCTGTCCTCCTGACAC
Collagenase 1 (MMP1)^∥	F: AGGTTATCCCAAAATGAT
Collagenase 1 (MMP1)^∥	R: TGCAGTTGAACCAGCTATT
Aggrecanase (MMP2)^§	F: AGGGCACCTCCTACAACAGC
Aggrecanase (MMP2)^§	R: CAGTGGACATAGCGGTCTCG
Collagenase 3 (MMP13)^∥	F: TTCTGGCACATGCTTTTCCTC
Collagenase 3 (MMP13)^∥	R: GGTTGGGGTCTTCATCTCCTG
TGF-β^†	F: CATCGATATGGAGCTGGTGAAG
TGF-β^†	R: GCCGTAATTTGGACAGGATCTG
TNF-α^†	F: CCTACCTGCTTCTCACCCATACC
TNF-α^†	R: TTGATGGCAGAGAGAAGGTTGA
IFN-γ^†	F: ATTTCGGTCAATGACGAGCAT
IFN-γ^†	R: GTTTCCTCTGGTTCGGTGACA
iNOS (LT)^¶	F: TGGATGCAACCCCATTGTC
iNOS (LT)^¶	R: CCCGCTGCCCCAGTTT

Primers designed by KSS. All amplicons were sequenced to confirm gene transcript specificity.

^†

Allen SS, et al. Infect Immun 2003;71(8):4271-7.

^‡

Yamada H, et al. Exp Anim 2005;54(2):163-172.

^§

Oh C, et al. Am J Obstet Gynecol 2008;199(1):78.e1-6.

^∥

Huebner JL, et al. Arthritis Rheum 1998;41(5):877-90.

^¶

Thompson L, et al. Pediatr Res 2009;65(2):188-92.